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IBT Protein Production, Characterization and Molecular Interaction (PPCMI)

Overview of Services

The Protein Production, Characterization and Molecular Interaction (PPCMI) core provides cloning, expression and purification services of functional proteins at a scale that meets the quantity and purity benchmarks for structural, biophysical, biochemical, and therapeutics studies; it also characterizes macromolecular biophysics and interaction energetics using a wide variety of state-of-the-art instrumentation and techniques that can analyze association and kinetic binding constants by surface plasmon resonance, enthalpies and entropies of binding by isothermal titration calorimetry, as well as determination of stoichiometry, stability, and homogeneity by both techniques.

Services:

  • Protein Production Services:
    • Guaranteed Bacterial Protein Expression Service Package: Customers just provide protein sequence (protein sequences are subject to pre-project analysis, and exclusions are applicable to membrane proteins, toxic proteins and target proteins larger than 1,000 amino acids) and our core will deliver the protein with the amount and purity which were specified by the customers. In case we fail on either the amount or purity as specified, there is no charge to the customer whatsoever.
    • Customized Protein Service Package / Gene-To-Protein Package: Recombinant protein production for a variety of difficult-to-express targets such as transmembrane proteins, toxins, receptors, and ion channel proteins may not be easy or straightforward; and usually the optimization process is labor tedious and time consuming since too many factors can affect protein expression and purification. To address some of the uncertainties and challenges associated with protein expression optimization and purification, the core facility offers cutting-edge solutions by leveraging its experience and expertise to provide customized bacterial expression services. Depending on project complexity, the delivery time can be as short as six weeks. This customized protein service, as a one-stop service platform from gene to protein, is ideal for challenging and difficult-to-express proteins, as well as flexible and affordable to meet customers’ demands.
    • Guaranteed Antibody Fragment Services: Antibody fragments, in particular scFvs, Fabs, and VHH/VH retain full antigen-binding capacity and superior properties for research, diagnostic, and therapeutic applications. We offer the guaranteed antibody fragment services that provide high-quality soluble antibody fragments at very competitive prices and timelines. Customers simply provide us with the protein sequence of the antibody fragment and we will take care of the whole process from gene synthesis to final purified antibody fragment.
    • Custom Cloning Services
    • E. coli Expression Test Services
    • Large Scale Protein Expression and Purification Services
    • Yeast Fermentation and Expression Services
  • Protein Interaction Services: The Core characterizes macromolecular biophysics and interaction energetics using a wide variety of state-of-the-art instrumentation and techniques that can analyze association and kinetic binding constants by surface plasmon resonance (SPR), enthalpies and entropies of binding by isothermal titration calorimetry (ITC), as well as determination of stoichiometry, stability, and homogeneity by both techniques.

Leadership

Magnus Hook, PhD | Director

Location and hours of operation

Hours Location

Monday - Friday

9am - 6pm                        

2121 W. Holcombe Blvd., Lab 613

Houston, TX 77030

Links and Resources

Contacts

Name Role Phone Email Location
Wen Liu
Manager/Protein Production
 
713-677-7583
 
wen_liu@tamu.edu
 
Rm 613
 
Kelly Churion
Manager/Protein Interaction
 
713-677-7608
 
franceschi@tamu.edu
 
Rm 611
 
Olivia Cabrera
Billing Administrator
 
713-677-7553
 
ocabrera@tamu.edu
 
Rm 604
 

Map


2121 W Holcombe Blvd, Houston, TX 77030