Welcome!
ALERT ATTENTION ⚠️
In Observance of U.S. Holiday, Independence Day, Agilent Cross Lab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Monday July 4th, 2022. We will resume regular U.S. support hours on Tuesday July 5th, 2022. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.
The Texas A&M Institute for Genome Sciences and Society (TIGSS) manages a molecular genomics laboratory with shared equipment to support genomics-based research at Texas A&M University. TIGSS offers training and experimental support for many technologies upstream of sequence generation. Equipment available for use includes the Fluidigm C1 single-cell system for extraction and isolation of nucleic acids and template preparation for sequencing or qPCR from single cells. The Fluidigm Biomark HD system for high-throughput real-time or end-point PCR in nanoliter volumes is also available for single cell analysis. Further equipment available for the generation of single cell sequencing libraries include the Eppendorf epMotion 5075 robot for library preparation, the Agilent Tape Station and BioTek Cytation 5 for library quality control, and the Illumina MiSeq or NextSeq 500 for generation of up to 400 million single end or 800 million paired end sequencing reads per run.
David Threadgill, TIGSS Director
Andrew Hillhouse, Molecular Genomics Workspace, Associate Director
| Hours | Location |
|
Monday - Friday 8:00 - 5:00 |
Texas A&M - University 206 Olsen Blvd 445 Reynolds Medical Building College Station, TX 77843 |
Resources available through the TIGSS shared molecular genomics laboratory can be found at:https://genomics.tamu.edu/shared-resources/
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Andrew Hillhouse |
Molecular Genomics Workspace, Associate Director
|
979-458-5678
|
hillhouse@tamu.edu
|
446A Reynolds Medical Building
|
| Price List |
| ► Full Service - Bioinformatics (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Bioinformatics Service (per hour) | Inquire | ||
| ► Full Service - Cell Counting (2) | |||
| Name | Description | Price | |
| Countess Usage Fee (per use) | Inquire | ||
| Countess Assay (per sample) | Inquire | ||
| ► Full Service - 10x (3) | |||
| Name | Description | Price | |
| 10x Sample Prep - Dead Cell Removal (per sample) |
The enrichment of live cells from single cell suspension using the Miltenyi Dead Cell Removal Kit |
Inquire | |
| 10x Single Cell mRNA Library Prep (per sample) |
Chromium Single Cell Gene Expression provides mRNA profiling at single cell resolution. Transcriptomes are analyzed on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) libraries. Specifically, single cells, reverse transcription (RT) reagents, Gel Beads containing barcoded oligonucleotides, and partitioning oil are combined on a microfluidic chip to form nanoliter-scale reaction vesicles. Within each reaction vesicle, a single cell is lysed, the Gel Bead is dissolved to free the identically barcoded RT oligonucleotides into solution, and reverse transcription of polyadenylated mRNA occurs. As a result, all cDNAs from a single cell will have the same barcode, allowing the sequencing reads to be mapped back to their single cells of origin. The preparation of NGS libraries from these barcoded cDNAs is then carried out in a highly efficient bulk reaction. |
Inquire | |
| 10x Single Cell ATAC Library Prep (per sample) |
Chromium Single Cell ATAC (Assay for Transposase Accessible Chromatin) allows you to analyze chromatin accessibility at the single cell level, providing insights into cell types and states, and deeper understanding of gene regulatory mechanisms. |
Inquire | |
| ► Full Service - Illumina iScan (5) | |||
| Name | Description | Price | |
| Infinium processing; Ultra HD 24x1 (192 samples) | Inquire | ||
| Infinium processing; Ultra HD 24x1 (96 samples) | Inquire | ||
| Infinium processing; Ultra HD 24x1 or 12x1 (48 samples) | Inquire | ||
| Infinium processing; Super HD 8x1 (48 samples) | Inquire | ||
| Infinium processing; HTS 24x1 (48 samples) | Inquire | ||
| ► Full Service - Illumina iSeq (1) | |||
| Name | Description | Price | |
| Read Length - 150x150 (4 million read pairs) | Inquire | ||
| ► Full Service - Illumina MiSeq (13) | |||
| Name | Description | Price | |
| Read Length - 250 SE (1 million reads) |
|
Inquire | |
| Read length-75x75 PE (1 million read pairs) | Inquire | ||
| Read length-150x150 PE (1 million read pairs) |
|
Inquire | |
| Read Length-250x250 PE (1 million read pairs) |
|
Inquire | |
| Read length-75x75 PE (4 million read pairs) | Inquire | ||
| Read length-150x150 PE (4 million read pairs) | Inquire | ||
| Read Length-50 SE (15 million reads) | Inquire | ||
| Read Length-250 SE (15 million reads) | Inquire | ||
| Read length-150x150 PE (15 million read pairs) | Inquire | ||
| Read length-250x250 PE (15 million read pairs) | Inquire | ||
| Read Length-150 SE (25 million reads) | Inquire | ||
| Read Length-75x75 PE (25 million read pairs) | Inquire | ||
| Read Length-300x300 PE (25 million read pairs) | Inquire | ||
| ► Full Service - Illumina NextSeq 500 (7) | |||
| Name | Description | Price | |
| Read Length - 150 SE (130 million reads) | Inquire | ||
| Read Length - 75x75 PE (130 million read pairs) | Inquire | ||
| Read Length - 150x150 PE (130 million read pairs) | Inquire | ||
| Read Length - 75 SE (400 million reads) | Inquire | ||
| Read Length - 150 SE (400 million reads) | Inquire | ||
| Read Length - 75x75 PE (400 million read pairs) | Inquire | ||
| Read Length - 150x150 PE (400 million read pairs) | Inquire | ||
| ► Full Service - Illumina NovaSeq (NTGC) (6) | |||
| Name | Description | Price | |
| S2 - Standard workflow (whole flow cell) 2x100 (3.3-4.1 billion clusters) | Inquire | ||
| S2 - Standard workflow (whole flow cell) 2x150 (3.3-4.1 billion clusters) | Inquire | ||
| S4 - XP workflow (individual lane) 2x100 (2-2.5 billion clusters) | Inquire | ||
| S4 - XP workflow (individual lane) 2x150 (2-2.5 billion clusters) | Inquire | ||
| S4 - Standard workflow (whole flow cell) 2x100 (8-10 billion clusters) | Inquire | ||
| S4 - Standard workflow (whole flow cell) 2x150 (8-10 billion clusters) | Inquire | ||
| ► Full Service - Oxford Nanopore (4) | |||
| Name | Description | Price | |
| Full Service - Flow Cell R10.3 |
R10.3 is our newest nanopore chemistry, providing improved homopolymer performance. It is suitable for experiments where high consensus accuracy is required. It can provide data with 1D accuracies up to 95% and consensus accuracies over Q50. As a new product it will be rapidly iterating, and it is currently only recommended for our DNA ligation sequencing chemistry. |
Inquire | |
| Full Service - Flow Cell R9.4.1 |
A MinION Flow Cell (R9.4.1) is a consumable used with MinION and GridION X5 devices. It contains the proprietary sensor array, Application-Specific Integrated Circuit (ASIC) and R9.4.1 nanopores suitable for all 1D experiments. |
Inquire | |
| Full Service - Ligation Sequencing Kit |
A versatile sequencing kit optimised for throughput. 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION |
Inquire | |
| Full Service - Rapid Barcoding Kit |
Simple and rapid library preparation, with barcoding for up to 12 gDNA samples 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION |
Inquire | |
| ► Full Service - Sample Preparation (7) | |||
| Name | Description | Price | |
| DNA isolation w/ FastDNA SPIN Kit (per sample) |
Protocol for DNA isolation from fecal pellets, soil, or other sources |
Inquire | |
| Gentra Puregene DNA Isolation (300uL blood) | Inquire | ||
| Maxwell 16 LEV simplyRNA Purification Kit (Tissue) |
Tissue 48 preps in one kit. |
Inquire | |
| Molecular Biology Lab Work (per hour) | Inquire | ||
| NEBNext PolyA mRNA Magnetic Isolation Module (per sample) |
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails. |
Inquire | |
| NucleoMag Plant DNA Isolation (per sample) |
Includes DNA extraction from 20-50 mg plant material and Qubit quantification. |
Inquire | |
| Trizol Total RNA Isolation (per sample) | Inquire | ||
| ► Full Service - NGS Library Preparation (13) | |||
| Name | Description | Price | |
| Arima HiC Library Prep (8 samples) | Inquire | ||
| BioO NEXTflex ChIP-Seq Library Prep (per sample) | Inquire | ||
| IDT xGen Amplicon Library Prep (per sample) |
Next Generation Sequenicng library preparation for metagenomic studies assaying bacterial V1-V9 variable regions and/or fungal ITS regions. |
Inquire | |
| IDT xGen Broad-Range RNA Library Prep (per sample) |
The Swift RNA Library Kit offers a fast, robust NGS transcriptomics workflow with optimal transcript coverage and NGS data quality from a broad range of input quantities for Illumina® sequencing platforms. Leveraging patented Adaptase® technology, this kit enables stranded RNA library construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. The kit is compatible with manual and automated workflows as well as upstream and downstream enrichment and depletion methods and supports a variety of indexing options. |
Inquire | |
| Illumina 16S rRNA Library Prep , V3-V4 Region (per sample) | Inquire | ||
| Illumina DNA (Flex) Library Prep (per sample) |
Formerly the Illumina Nextera DNA Flex Kit, this has transitioned to the Illumina DNA (M) Tagmentation Kit. |
Inquire | |
| Illumina Free Adapter Blocking (per sample) | Inquire | ||
| Illumina Nextera XT Library Prep (per sample) | Inquire | ||
| Illumina TruSeq DNA PCR-Free Library Prep (per sample) | Inquire | ||
| Illumina TruSeq Stranded mRNA Library Prep (per sample) | Inquire | ||
| Illumina TruSeq Stranded Total RNA with Ribo-Zero (species specific) Library Prep (per sample) | Inquire | ||
| PerkinElmer NEXTflex Rapid DNA Library Prep (per sample) | Inquire | ||
| PerkinElmer NextFlex Small RNA Library Prep (per sample) | Inquire | ||
| ► Full Service - Qubit (1) | |||
| Name | Description | Price | |
| RNA/DNA Quantification Assay (per sample) | Inquire | ||
| ► Full Service - TapeStation/Bioanalyzer (14) | |||
| Name | Description | Price | |
| TapeStation DNA D1000 (1-16 samples) | Inquire | ||
| TapeStation DNA HS D1000 (1-16 samples) | Inquire | ||
| TapeStation DNA D1000 (17-96 samples) | Inquire | ||
| TapeStation DNA HS D1000 (17-96 samples) | Inquire | ||
| TapeStation DNA D5000 (1-16 samples) | Inquire | ||
| TapeStation DNA HS D5000 (1-16 samples) | Inquire | ||
| TapeStation DNA D5000 (17-96 samples) | Inquire | ||
| TapeStation DNA HS D5000 (17-96 samples) | Inquire | ||
| TapeStation Genomic DNA (1-16 samples) | Inquire | ||
| TapeStation Genomic DNA (17-96 samples) | Inquire | ||
| TapeStation RNA Standard (1-16 samples) | Inquire | ||
| TapeStation RNA HS (1-16 samples) | Inquire | ||
| TapeStation RNA Standard (17-96 samples) | Inquire | ||
| TapeStation RNA HS (17-96 samples) | Inquire | ||
| ► Self Service - Bio-Plex 200 (1) | |||
| Name | Description | Price | |
| Bio-Plex 200 Usage Fee (per use) - Self | Inquire | ||
| ► Self Service - Cell Counting (1) | |||
| Name | Description | Price | |
| Countess Assay (per sample) - Self | Inquire | ||
| ► Self Service - Cytation 5 (1) | |||
| Name | Description | Price | |
| Cytation 5 Usage Fee (per use) - Self | Inquire | ||
| ► Self Service - epMotion 5075 (1) | |||
| Name | Description | Price | |
| epMotion Usage Fee (per use) - Self | Inquire | ||
| ► Self Service - ddPCR (1) | |||
| Name | Description | Price | |
| ddPCR Probe Chemistry (per sample) - Self |
Must run multiples of 8. |
Inquire | |
| ► Self Service - Qubit (1) | |||
| Name | Description | Price | |
| RNA/DNA Quantification Assay (per sample) - Self |
Broad range and high sensitivity dsDNA and RNA assays. |
Inquire | |
| ► Self Service - TapeStation/Bioanalyzer (13) | |||
| Name | Description | Price | |
| DNA D1000 (1-16 samples) - Self | Inquire | ||
| DNA HS D1000 (1-16 samples) - Self | Inquire | ||
| DNA D1000 (17-96 samples) - Self | Inquire | ||
| DNA HS D1000 (17-96 samples) - Self | Inquire | ||
| DNA D5000 (1-16 samples) - Self | Inquire | ||
| DNA HS D5000 (1-16 samples) - Self | Inquire | ||
| DNA D5000 (17-96 samples) - Self | Inquire | ||
| DNA HS D5000 (17-96 samples) - Self | Inquire | ||
| Genomic DNA (1-16 samples) - Self | Inquire | ||
| Genomic DNA (17-96 samples) - Self | Inquire | ||
| RNA HS (1-16 samples) - Self | Inquire | ||
| RNA Standard (17-96 samples) - Self | Inquire | ||
| RNA HS (17-96 samples) - Self | Inquire | ||
